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Thermo Fisher
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Cell Signaling Technology Inc
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GeneTex
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Millipore
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Cell Signaling Technology Inc
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Santa Cruz Biotechnology
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Santa Cruz Biotechnology
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Cell Signaling Technology Inc
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Image Search Results
Journal: Biomolecules & Therapeutics
Article Title: Cellular Prion Protein Enhances Drug Resistance of Colorectal Cancer Cells via Regulation of a Survival Signal Pathway
doi: 10.4062/biomolther.2017.033
Figure Lengend Snippet: The effect of PrP C on cell stress-associated protein in colorectal cancer cells. (A) The phosphorylation of p38 in SNU-C5/5FUR cells transfected with si-scr or si- PRNP after treatment with PBS or 5-FU (140 μM) for 48 h. (B) The level of p-p38 was determined by densitometry, relative to total p38 expression. (C) The phosphorylation of JNK in SNU-C5/5FUR cells transfected with si-scr or si- PRNP after treatment with PBS or 5-FU (140 μM) for 48 h. (D) The level of p-JNK was determined by densitometry, relative to total JNK expression. (E) The phosphorylation of p53 in SNU-C5/5FUR cells transfected with si-scr or si- PRNP after treatment with PBS or 5-FU (140 μM) for 48 h. (F) The level of p-p53 was determined by densitometry, relative to total p53 expression. (G) The phosphorylation of ATM in SNU-C5/5FUR cells transfected with si-scr or si- PRNP after treatment with PBS or 5-FU (140 μM) for 48 h. (H) The level of p-ATM was determined by densitometry, relative to total ATM expression. (B, D, F, H) Data are expressed as the mean ± SEM of three independent experiments. ** p <0.01 vs. scrambled siRNA-transfected SNU-C5/5FUR (si-scr) treated with PBS, $$ p <0.01 vs. si- PRNP treated with PBS, and ## p <0.01 vs. si-scr treated with 5-FU.
Article Snippet: The membranes were blocked with 5% skim milk and incubated with primary antibodies against PrP C , phospho-phosphatidylinositol-3-kinase (PI3K), total PI3K, phospho-AKT, total AKT, cyclin dependent kinase (CDK) 2, CDK4, cyclin D1, cyclin E, phosphor-p38, total p38, phosphor-c-JUN N-terminal kinase (JNK), total JNK, cleaved caspase-3, cleaved poly [ADP-ribose] polymerase 1 (PARP1), β-actin (Santa Cruz Biotechnology, Dallas, TX, USA), phospho-p53, total p53 (Cell Signaling Technology, Danvers, MA, USA), phospho-ataxia-telangiectasia mutated (ATM), and
Techniques: Transfection, Expressing
Journal: Cancer cell
Article Title: Inhibition of Nuclear PTEN Tyrosine Phosphorylation Enhances Glioma Radiation Sensitivity through Attenuated DNA Repair
doi: 10.1016/j.ccell.2019.04.011
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Recombinant, Mutagenesis, Transfection, Fractionation, Cell Culture, Negative Control, Plasmid Preparation, Construct, Software